Immunohistochemical Study of Matrix Metalloproteinase-9 in Medial Collateral Ligament Epiligament in Rat Knee after Grade III Injury

  • Alexandar Iliev Department of Anatomy, Histology, and Embryology, Medical University of Sofia, Bulgaria
  • Georgi P. Georgiev Department of Orthopaedics and Traumatology, University Hospital Queen Giovanna-ISUL, Medical University Sofia, Bulgaria
  • Georgi Kotov Department of Anatomy, Histology, and Embryology, Medical University of Sofia, Bulgaria
  • Iva N. Dimitrova Department of Cardiology, University Hospital “St. Ekaterina”, Medical University of Sofia, Bulgaria
  • Lina Malinova Department of Anatomy, Histology, and Embryology, Medical University of Sofia, Bulgaria
  • Pavel Rashev Department of Immunology of Reproduction, Institute of Biology and Immunology of Reproduction – “Acad. K. Bratanov”, Bulgarian Academy of Sciences, Sofia, Bulgaria
  • Lubomir Tsankov Department of Image Diagnostic, University Hospital "Dr. G. Stransky", Medical University of Pleven, Bulgaria
  • Vesselin Karabinov MHAT National Cardiology Hospital, Clinics of Neurology, Sofia, Bulgaria
  • Boycho Landzhov Department of Anatomy, Histology, and Embryology, Medical University of Sofia, Bulgaria
Keywords: Matrix metalloproteinase-9, medial collateral ligament epiligament, grade III injury, immunohistochemical study

Abstract

Background: In the present study, we conduct an immunohistochemical analysis to evaluate the expression of matrix metalloproteinase-9 (MMP-9) in the epiligament (EL) after grade III injury of the medial collateral ligament (MCL) in an experimental model of a rat knee. Methods: Twelve 8-month old male Wistar rats were used in this study. Three animals were used as controls, while the remaining nine underwent grade III injury of the MCL. The MMP-9 immunoreactivity was evaluated on the 8th, 16th and 30th day after injury. Results: We observed an intensive expression of the enzyme in all periods after injury in contrast with the control group. We also discovered that the main source of matrix metalloproteinase-9 was localized in the epiligament tissue. Immunoreactivity was highest and homogeneously distributed on the 8th day and gradually diminished, concentrating on the EL-ligament border and the perivascular zones on the 30th day. Conclusion: We present the first immunohistochemical study of the expression and distribution of the enzyme MMP-9 in the EL of the MCL and track the changes in enzyme activity on the 8th, 16th and 30th day after damage.

Published
2017-06-15
How to Cite
Iliev, A., Georgiev, G. P., Kotov, G., Dimitrova, I. N., Malinova, L., Rashev, P., Tsankov, L., Karabinov, V., & Landzhov, B. (2017). Immunohistochemical Study of Matrix Metalloproteinase-9 in Medial Collateral Ligament Epiligament in Rat Knee after Grade III Injury. Academia Anatomica International, 3(1), 20-25. https://doi.org/10.21276/aanat.2017.3.1.6